MoxT toxin of Bacillus anthracis exhibits sequence specific ribonuclease activity

作者：Verma Shashikala^*; Bhatnagar Rakesh

来源：Biochemical and Biophysical Research Communications, 2014, 450(2): 998-1004.

DOI：10.1016/j.bbrc.2014.06.092

• Bacillus anthracis
• MoxT
• Ribonuclease
• RNA sequence

MoxXT module of Bacillus anthracis encodes MoxX, a labile protein and MoxT, a ribonuclease. However, mechanism of cleavage of RNA by MoxT has not been explored till date. In the present study, we have demonstrated that MoxT is a sequence specific ribonuclease which recognizes UACAU sequence in ss RNA and cleaves between U and A. Moreover, cleavage of RNA requires 2' OH group of first residue, i.e. U of UACAU RNA sequence. An interesting finding which makes it distinct from the other MazF family toxins was also observed, i.e. its ability to cleave RNA in DNA RNA hybrid.

• 出版日期2014-7-25