科研之友
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摘要
alpha-phellandrene (alpha-PA) is a cyclic monoterpene, present in natural plants such as Schinus molle L. alpha-PA promotes immune responses in mice in vivo. However, there is no available information on whether alpha-PA affects gene expression in leukemia cells. The present study determined effects of alpha-PA on expression levels of genes associated with DNA damage, cell cycle and apoptotic cell death in mouse leukemia WEHI-3 cells. WEHI-3 cells were treated with 10 ktM alpha-PA for 24 h, cells were harvested and total RNA was extracted, and gene expression was analyzed by cDNA microarray. Results indicated that alpha-PA up-regulated 10 genes 4-fold, 13 by over 3-fold and 175 by over 2-fold; 21 genes were down-regulated by over 4-fold, 26 genes by over 3-fold and expression of 204 genes was altered by at leas 2-fold compared with the untreated control cells. DNA damage-associated genes such as DNA damage-inducer transcript 4 and DNA fragmentation factor were upregulated by 4-fold and over 2-fold, respectively; cell-cycle check point genes such as cyclin G2 and cyclin-dependent kinases inhibitor 2D and IA (p21) were up-regulated by over 3-fold and over 2-fold, respectively; apoptosis-associated genes such as BCL2ladenovirus EIB interacting protein 3, XIAP-associated factor 1, BCL2 modifying factor, caspase8 and FADD-like apoptosis regulator were over 2-fold up- regulated. Furthermore, DNA damage-associated gene TATA box binding protein was over 4-fol4 down-regulated, and D19Ertd652c (DNA segment) over 2-fold down-regulated; cell cycle-associated gene cyclin E2 was over 2-fold downregulated; apoptosis associated gene growth arrest-specific 5 was over 9-fold down-regulated, Gm5426 (ATP synthase) was over 3-fold down-regulated, and death box polyp eptide 33 was over 2-fold down-regulated. Based on these observations, alpha-PA altered gene expression in WEHI-3 cells in vitro.
- 出版日期2014-8
- 单位中国医科大学
