A phage protein that binds phi C31 integrase to switch its directionality

作者：Khaleel Thanafez; Younger Ellen; McEwan Andrew R; Varghese Anpu S; Smith Margaret C M^*

来源：Molecular Microbiology, 2011, 80(6): 1450-1463.

DOI：10.1111/j.1365-2958.2011.07696.x

The serine integrase, Int, from the Streptomyces phage phi C31 mediates the integration and excision of the phage genome into and out of the host chromosome. Integrases usually require a recombination directionality factor (RDF) or Xis to control integration and excision and, as phi C31 Int only mediates integration in the absence of other phage proteins, we sought to identify a phi C31 RDF. Here we report that the phi C31 early protein, gp3 activated attL x attR recombination and inhibited attP x attB recombination. Gp3 binds to Int in solution and when Int is bound to the attachment sites. Kinetic analysis of the excision reaction suggested that gp3 modifies the interactions between Int and the substrates to form an active recombinase. In the presence of gp3, Int assembles an excision synaptic complex and the accumulation of the integration complex is inhibited. The structure of the excision synaptic complex, like that of the hyperactive mutant of Int, IntE449K, appeared to be biased towards one that favours the production of correctly joined products. The functional properties of phi C31 gp3 resemble those of the evolutionarily unrelated RDF from phage Bxb1, suggesting that these two RDFs have arisen through convergent evolution.

• 出版日期2011-6
• 单位河北医科大学