Nitric oxide and hydrogen peroxide are involved in indole-3-butyric acid-induced adventitious root development in marigold

作者：Liao, Weibiao^*; Huang, Gaobao; Yu, Jihua; Zhang, Meiling; Shi, Xueliang

来源：Journal of Horticultural Science & Biotechnology, 2011, 86(2): 159-165.

DOI：10.1080/14620316.2011.11512742

• INDUCED STOMATAL CLOSURE
• RESPONSES
• SUPEROXIDE
• ELONGATION
• SENESCENCE
• RELEASE
• CALCIUM
• LIGHT

Previous results have shown that both nitric oxide (NO) and hydrogen peroxide (H2O2) play crucial roles in the promotion of adventitious root development in explants of marigold (Tagetes erecta L.). In this study, the involvement of NO and H2O2 in indole-3-butyric acid (IBA)-induced adventitious root development in marigold explants, and the order of action of these molecules in the signal transduction pathway were investigated. The results indicated that, like NO and H2O2, IBA-induced adventitious rooting occurred in a dose-dependent manner. Two parameters of root growth in explants treated simultaneously with IBA and H2O2 were significantly higher than those in explants treated with IBA or H2O2 alone, suggesting that IBA and H2O2 may act synergistically to mediate adventitious rooting. NO and H2O2 were pre-requisites for the adventitious rooting induced by IBA. Moreover, treatment with IBA enhanced the endogenous levels of NO and H2O2 in explants, indicating that the induction of adventitious roots by IBA occurred through enhancing the levels of NO and H2O2. NO and H2O2 may be downstream signal molecules in the auxin signalling cascade, and NO may be involved as an upstream signalling molecule for H2O2 production.

• 出版日期2011-3
• 单位甘肃农业大学