A cyclic enzymatic amplification method for sensitive and selective detection of nucleic acids

作者：Cui, Liang; Ke, Guoliang; Wang, Chunming; Yang, Chaoyong James^*

来源：Analyst, 2010, 135(8): 2069-2073.

DOI：10.1039/c0an00215a

• SIGNAL AMPLIFICATION
• NICKING ENDONUCLEASE
• DNA
• PROBES
• ASSAY
• SEQUENCE
• GENERATION
• HELICASE
• PROTEIN

Based on Exonuclease III (Exo III) and displacing probes, we have developed a Cyclic Enzymatic Amplification Method (CEAM) for sensitive and selective detection of nucleic acids. In this design, the displacing probe is non-fluorescent on its own and cannot be digested by Exo III until displacement hybridization by a target sequence, leading to release of free non-quenched fluorophore. Because a single target sequence can lead to the release and digestion of numerous fluorophore strands from the displacing probe, a remarkable signal amplification is achieved. With this method, DNA can be detected in the picomolar range with a high selectivity and within less than 20 min.

• 出版日期2010
• 单位厦门大学