Binding interaction between aloe polysaccharide and alizarin red by spectrophotometry and its analytical application

作者：Gu Wenxiu^*; Song Hongyan; Wen Xiaonan; Wang Yingying; Xia Wenshui; Fang Yun

来源：Carbohydrate Polymers, 2010, 80(1): 115-122.

DOI：10.1016/j.carbpol.2009.10.074

• Alizarin red
• Aloe polysaccharide
• Spectrophotometry

In order to aid the detection of and to reveal the physiological function of aloe polysaccharide (APS), spectroscopic Studies of aloe polysaccharide and alizarin red (AR) interaction are presented in this work. APS was identified as all acetylated mannan, which consists of a polydispersed beta- 1,4-linked mannan substituted with O-acetyl groups. The binding interaction between APS and AR was investigated by experiments and theoretical calculation, respectively. The experimental Value is in good agreement with the theoretical one. The results show that co-action of the electrostatic and hydrophobic interactions between AIR and APS contributes to APS-AR complex chromogenic reaction. Based on this interaction, a new method for spectrophotometric determination of APS with AR is proposed. The maximum absorbances of the complex are at 325 rim and 516 nm. The corresponding linear equations are A = 0.0109 rho + 0.0441, R(2) = 0.9992 and A = 0.0121 rho + 0.0288, R(2) = 0.9988. The linear range is 0-60.00 mu g/mL and the average recovery is 100.2 +/- 5%, RSD <= 5%.

• 出版日期2010-3-25
• 单位江南大学