Novel insights into the use of Glowing LNA as nucleic acid detection probes-Influence of labeling density and nucleobases

作者：Ostergaard Michael E; Maity Jyotirmoy; Babu B Ravindra; Wengel Jesper; Hrdlicka Patrick J^*

来源：Bioorganic & Medicinal Chemistry Letters, 2010, 20(24): 7265-7268.

DOI：10.1016/j.bmcl.2010.10.089

• Oligonucleotides
• Pyrene
• Locked nucleic acid (LNA)
• Conformationally restricted nucleosides
• RNA imaging
• Hybridization probes
• Nucleic acid diagnostics
• Nucleic acid targeting

Appropriately designed 2'-N-(pyren-1-yl)carbonyl-2'-amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets. Thermal denaturation and fluorescence spectroscopy studies on ONs modified with known thymine monomer X and novel 5-methylcytosine monomer Y provide new insights into the design principles and mechanism of these Glowing LNA nucleic acid detection probes. Published by Elsevier Ltd.

• 出版日期2010-12-15