科研之友
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摘要
Objective-To evaluate the effects of IM administration of recombinant human thyroid-stimulating hormone (rhTSH) on plasma total thyroxine (T-4) concentrations in euthyroid ferrets. %26lt;br%26gt;Design-Evaluation study. %26lt;br%26gt;Animals-25 healthy neutered ferrets (14 female and 11 male) of various ages from 2 populations (laboratory ferrets from Georgia and pet ferrets from Pennsylvania). %26lt;br%26gt;Procedures-Each ferret underwent a physical examination and standard hematologic testing to ensure it was healthy and had clinically normal thyroid function. Once determined to be euthyroid, ferrets received a single IM injection of 100 jig of rhTSH. Blood samples were collected into plasma-separator tubes immediately before the rhTSH injection (time 0) and 4 hours after injection to measure T-4 concentrations. %26lt;br%26gt;Results-Males did not differ from females in regard to prestimulation or poststimulation plasma T-4 concentrations; however, prestimulation and poststimulation T-4 concentrations were significantly different between the 2 groups of ferrets. A significant difference was also identified between prestimulation T-4 concentration (mean +/- SD, 21.3 +/- 6.1 nmol/L) and poststimulation T-4 concentration (29.9 +/- 8.2 nmol/L). All 25 ferrets had high poststimulation T-4 concentrations (median difference, 7.5 nmol/L; 10% to 90% interval, 3.26 to 17.70 nmol/L [0.25 to 1.38 mu g/dL]; range, 2.50 to 20.70 nmol/L [0.19 to 1.61 mu g/dL]); this represented a median increase in T-4 concentration of 35% (10% to 90% interval, 18% to 81%; range, 8% to 126%). %26lt;br%26gt;Conclusions and Clinical Relevance-Results suggested that rhTSH can be used for thyrotropin stimulation testing in ferrets when administered IM. According to the findings, a euthyroid ferret should have an increase of approximately 30% in plasma T-4 concentration 4 hours after rhTSH injection.
- 出版日期2013-11-15
