The vanilloid capsaicin induces IL-6 secretion in prostate PC-3 cancer cells

作者：Malagarie Cazenave Sophie^*; Olea Herrero Nuria; Vara Diana; Morell Cecilia; Diaz Laviada Ines

来源：Cytokine, 2011, 54(3): 330-337.

DOI：10.1016/j.cyto.2011.03.010

• Capsaicin
• IL-6
• TNF-alpha
• PC-3 cells
• Prostate cancer

Capsaicin (8-methyl-N-vanillyl-6-nonenamide), a constituent of green and red peppers, has been linked with suppression of tumorigenesis through a mechanism that is not well understood. In the present study, we examined the effects of capsaicin on the production of the cytokine interleukin (IL)-6 by PC-3 cells at both protein and mRNA levels which were evaluated by ELISA and real-time PCR, respectively. Capsaicin-treated PC-3 cells increased the synthesis and secretion of IL-6 which was abrogated by the transient receptor potential vanilloid receptor subtype 1 (TRPV1) antagonist capsazepine, as well as by inhibitors of PKC-alpha, phosphoinositol-3 phosphate kinase (PI-3K), Akt and extracellular signal-regulated protein kinase (ERK). We analyzed the role of capsaicin in the tumor necrosis factor (TNF)-alpha secretion by PC-3 cells which was increased at shorter times than IL-6 production. Furthermore, incubation of PC-3 cells with an anti-TNF-alpha antibody blocked the capsaicin-induced IL-6 secretion. These results raise the possibility that capsaicin-mediated IL-6 increase in prostate cancer PC-3 cells is regulated at least in part by TNF-alpha secretion and signaling pathway involving Akt, ERK and PKC-alpha activation.

• 出版日期2011-6