Determination of internal transcribed spacer regions (ITS) in Trichomonas vaginalis isolates and differentiation among Trichomonas species

作者：Ibanez Escribano Alexandra^*; Nogal Ruiz Juan Jose; Aran Vicente J; Escario Jose Antonio; Gomez Barrio Alicia; Alderete J F

来源：Parasitology International, 2014, 63(2): 427-431.

DOI：10.1016/j.parint.2013.12.017

• Trichomonas vaginalis
• rDNA
• Internal transcribed spacers (ITS)
• Protozoa
• Phylogenetic study
• Clades

The nucleotide sequence of the 5.8S rRNA gene and the flanked internal transcribed spacer (ITS) regions of six Trichomonas vaginalis isolates with different metronidazole sensitivity and geographic origin were genotyped. A multiple sequence alignment was performed with different sequences of other isolates available at the GenBank/EMBL/DDBJ databases, which revealed 5 different sequence patterns. Although a stable mutation in position 66 of the ITS1 (C66T) was observed in 26% (9/34) of the T. vaginalis sequences analyzed, there was 99.7% ITS nucleotide sequence identity among isolates for this sequence. The nucleotide sequence variation among other species of the genus Trichomonas ranged from 3.4% to 9.1%. Surprisingly, the % identity between T. vaginalis and Pentatrichomonas hominis was similar to 83%. There was %26gt; 40% divergence in the ITS sequence between T. vaginalis and Tritrichomonas spp., including Tritrichomonas augusta,Tritrichomonas mulls, and Tritrichomonas nonconforma and with Tetratrichomonas prowazeki. Dendrograms grouped the trichomonadid sequences in robust clades according to their genera. The absence of nucleotide divergence in the hypeivariable ITS regions between T. vaginalis isolates suggests the early divergence of the parasite. Importantly, these data show this ITS1-5.85 rRNA-ITS2 region suitable for inter-species differentiation.

• 出版日期2014-4