A combination study of spin-trapping, LC/ESR and LC/MS on carbon-centred radicals formed from lipoxygenase-catalysed peroxidation of eicosapentaenoic acid

作者：Shan Zhen; Yu Qingfeng; Purwaha Preeti; Guo Bin; Qian Steven Y^*

来源：Free Radical Research, 2009, 43(1): 13-27.

DOI：10.1080/10715760802567606

• ESR spin-trapping
• EPA-derived radicals
• LOX-catalysed peroxidation
• on-line LC
• ESR
• on-line LC
• MS and LC
• MS2

Increased evidence from animal and in vitro cellular research indicates that the metabolism of eicosapentaenoic acid (EPA) can inhibit carcinogenesis in many cancers. Free radical-mediated peroxidation is one of many possible mechanisms to which EPA's anti-cancer activity has been attributed. However, no direct evidence has been obtained for the formation of any EPA-derived radicals. In this study, a combination of LC/ESR and LC/MS was used with -[4-pyridyl 1-oxide]-N-tert-butyl nitrone to identify the carbon-centred radicals that are formed in lipoxygenase-catalysed EPA peroxidation. Of the numerous EPA-derived radicals observed, the major products were those stemming from -scission of 5-, 15- and 18-EPA-alkoxyl radicals. By means of an internal standard in LC/MS, this study also quantified each radical adduct in all its redox forms, including an ESR-active form and two ESR-silent forms. The comprehensive profile of EPA's radical formation provides a starting point for ongoing research in defining the biological effects of radicals generated from EPA peroxidation.

• 出版日期2009
• 单位中国药科大学