Associations of atherosclerotic risk factors with oxidized low-density lipoprotein evaluated by LOX-1 ligand activity in healthy men

作者：Uchida Kagehiro; Suehiro Akira; Nakanishi Mamoru; Sawamura Tatsuya; Wakabayashi Ichiro^*

来源：Clinica Chimica Acta, 2011, 412(17-18): 1643-1647.

DOI：10.1016/j.cca.2011.05.022

• Atherosclerosis
• Lectin-like oxidized LDL receptor
• Metabolic syndrome
• Oxidized low-density lipoprotein
• Smoking
• Triglycerides

Background: The aim of this study was to determine the relationships of risk factors for atherosclerosis with oxidized low-density lipoprotein (OxLDL) evaluated by a new enzyme immunoassay for measurement of LOX-1 (lectin-like OxLDL receptor) ligand.
Methods: Subjects were 236 healthy men aged 33-62 years. LOX-1 ligand containing apoB (LAB) was measured by an enzyme-immunoassay using immobilized recombinant LOX-1 and anti-ApoB monoclonal antibody.
Results: In simple regression analysis, log-converted LAB showed significant positive correlations with history of smoking, waist circumference, diastolic blood pressure, LDL cholesterol, log-converted triglycerides, uric acid and white blood cell count and showed a significant negative correlation with HDL cholesterol. In multiple regression analysis using history of smoking, history of drinking, waist circumference, diastolic blood pressure, HDL cholesterol, log-converted triglycerides and uric acid as explanatory variables, log-converted LAB showed significant correlations only with history of smoking and log-converted triglycerides. Log-converted LAB was significantly higher in heavy smokers (>= 20 cigarettes per day) than in nonsmokers and light smokers (<20 cigarettes per day), while no difference in log-converted LAB was found between nonsmokers and light smokers. Log-converted LAB was significantly higher in subjects with hypertriglyceridemia (>= 150 mg/dl), large waist circumference (>= 85 cm), high diastolic blood pressure (>= 85 mm Hg), or metabolic syndrome defined by the NCEP-ATP III criteria than in subjects without each risk factor or metabolic syndrome.
Conclusions: Hypertriglyceridemia and smoking are determinants of LOX-1 ligand activity in healthy men and are thus thought to be crucial risk factors for initiation of atherosclerotic progression through generation of OxLDL.

• 出版日期2011-8-17