A modified ferrous oxidation-xylenol orange assay for lipoxygenase activity in rice grains

作者：Timabud Tarinee; Sanitchon Jirawat; Pongdontri Paweena^*

来源：Food Chemistry, 2013, 141(3): 2405-2411.

DOI：10.1016/j.foodchem.2013.05.037

• Ferrous oxidation-xylenol orange (FOX) assay
• Lipoxygenase
• Hydroperoxy fatty acid product
• Rice grain

Ferrous oxidation-xylenol orange assay reagent was reformulated by using spectral analysis of ferricxylenol orange complex to detect low concentrations of lipoxygenase rice grain products. Reducing the levels of ferrous sulphate and xylenol orange in the FOX reagent enabled the detection of low concentrations of hydroperoxy fatty acid derived from lipoxygenase activity in the range of 0.1-1.5 mu M. Protein, substrate and time courses of the modified FOX assay were studied to determine lipoxygenase activity in rice grain. The assay was also applicable as a high throughput technique for comparisons of lipoxygenase activity from various rice varieties. This has important implications for rapid screening for low-lipoxygenase containing rice cultivars in rice breeding program and grain quality during storage.

• 出版日期2013-12-1