Astragalus Oral Solution Ameliorates Allergic Asthma in Children by Regulating Relative Contents of CD4(+)CD25(high)CD127(low) Treg Cells

作者：Wang, Wei^*; Jing, Wei; Liu, Qingbin

来源：Frontiers in Pediatrics, 2018, 6: 255.

DOI：10.3389/fped.2018.00255

• Astragalus oral solution
• Astragaloside A
• allergic asthma
• CD4(+)CD25(high)CD127(low) Treg cells
• children

Objective: To explore the effects of Astragalus oral solution (AOS) on allergic asthma in children by investigating relative contents of CD4(+)CD25(high)CD127(low) Treg cells.
Methods: The contents of Astragaloside A in AOS were detected by using HPLC. Eighty children with allergic asthma were recruited from February 2016 to June 2017, and randomly assigned into the control group (received placebo, 0.1% quinine chloride in deionized water, daily) and the AOS group (received 10mL AOS daily). After 6-month treatment, therapeutic results were compared between the two groups. Serum levels of IL-10 and TGF-beta, Th1 cytokines (IL-2 and IFN-gamma), and Th2 cytokines (IL-4 and IL-6) were measured by using ELISA kits. Relative contents of CD4(+)CD25(high)CD127(low) Treg cells were determined by using flow cytometry.
Results: Astragaloside A was the main ingredient of AOS with 0.216 +/- 0.027 mg/mL from six-batch samples. After 6-month therapy, the AOS group showed improved forced expiratory volume in 1 s (FEV1) and the Pediatric Asthma Quality of Life Questionnaire (PAQLQ) scores compared with the control group (P < 0.05). Serum level of IL-10 was higher and the levels of TGF-beta, Th1 cytokines (IL-2 and IFN-gamma), and Th2 cytokines (IL-4 and IL-6) were lower in the AOS group than in the control group (P < 0.05). AOS treatment increased the percentage of gated CD4(+) T cells, CD4(+)CD25(+) T cells, CD4(+)CD25(high) Treg cells, CD4(+)CD25(+)FoxP3(+) Treg cells and CD4(+)CD25(high)CD127(low) Treg cells when compared with the control group (P < 0.05).
Conclusions: Astragaloside A was the main component of AOS, and AOS ameliorated allergic asthma in children by regulating relative contents of CD4(+)CD25(high)CD127(low) Treg cells.

• 出版日期2018-9-20
• 单位长春中医药大学