A sensitive LC-MS/MS method for the quantification of urinary 8-iso-prostaglandin F-2 alpha (8-iso-PGF(2 alpha)) including pediatric reference interval

作者：Xiao Yi; Fu Xiaowei^*; Pattengale Paul; Bard Jennifer Dien; Xu Yan Kang; O'Gorman Maurice R

来源：Clinica Chimica Acta, 2016, 460: 128-134.

DOI：10.1016/j.cca.2016.06.034

• 8-iso-Prostaglandin F-2 alpha
• 8-iso-PGF(2 alpha)
• Pediatric reference interval
• Liquid chromatography-mass spectrometry

Background: Oxidative stress has been implicated in numerous diseases, including arthritis, atherosclerosis, Alzheimer's disease, cancer, diabetes, hypertension, and inflammation. 8-iso-prostaglandin F-2 alpha, a member of the F2 isoprostane family, has been well-accepted as a valuable biomarker for the assessment of oxidative stress.
Methods: We report the development and validation of an ultra-sensitive LC-MS/MS assay for urinary 8-iso-PGF(2 alpha) measurements in pediatric population.
Results: The assay was linear from 0.024 to 20 nmol/l (R-2 = 0.99). Recoveries were above 85% and matrix effects were below 5%. The variability was determined at nmol/l concentration: the intra-day variability (%CV) ranged from 3.9% to 4.5% (n = 20); and the inter-day variability ranged from 4.3% to 5.7% (n = 20). The accuracy of our laboratory developed test was evaluated with a clinical reference laboratory (n = 39), and a correlation coefficient of 0.9257 was observed. Reference interval were established to be <0.5 ng/mg creatinine in a group of pediatric population (2 months-18 years, n = 123).
Conclusions: The precision of the assay will allow for accurate assessment of oxidative stress, and is acceptable for patient testing, particularly in pediatric population.

• 出版日期2016-9-1