An engineered eukaryotic protein glycosylation pathway in Escherichia coli

作者：Valderrama Rincon Juan D^*; Fisher Adam C; Merritt Judith H; Fan Yao Yun; Reading Craig A; Chhiba Krishan; Heiss Christian; Azadi Parastoo; Aebi Markus; DeLisa Matthew P

来源：Nature Chemical Biology, 2012, 8(5): 434-436.

DOI：10.1038/NCHEMBIO.921

We performed bottom-up engineering of a synthetic pathway in Escherichia coli for the production of eukaryotic trimannosyl chitobiose glycans and the transfer of these glycans to specific asparagine residues in target proteins. The glycan biosynthesis was enabled by four eukaryotic glycosyltransferases, including the yeast uridine diphosphate-N-acetylglucosamine transferases Alg13 and Alg14 and the mannosyltransferases Alg1 and Alg2. By including the bacterial oligosaccharyltransferase PglB from Campylobacter jejuni, we successfully transferred glycans to eukaryotic proteins.

• 出版日期2012-5