Antiviral properties of polysaccharides from Agaricus brasiliensis in the replication of bovine herpesvirus 1

作者：Minari M C; Rincao V P; Soares S A; Ricardo N M P S; Nozawa C; Linhares R E C^*

来源：Acta Virologica, 2011, 55(3): 255-259.

DOI：10.4149/av_2011_03_255

• bovine herpesvirus 1
• Agaricus brasiliensis
• antiviral activity

Natural products are an inexhaustible source of compounds with promising pharmacological activities, including antiviral action. In the present study, the antiviral potential of polysaccharide-peptide (PLS) and an extracted beta-glucan from Agaricus brasiliensis were investigated in the replication of bovine herpesvirus 1 (BoHV-1) in HEp-2 cell cultures. The cytotoxicity (CC(50)) was assayed by the MTT method and the antiviral activity (IC(50)) was estimated by the plaque reduction assay. To study the possible mode of action of PLS and beta-glucan, the following protocols were performed: the virucidal assay, adsorption assay and the time-of-addition assay. The PLS presented a selectivity index (SI) higher than 12.50 and beta-glucan 9.19. The antiviral inhibition (67.9%) in cells treated with PLS during virus infection was higher than that in cells treated prior to or post infection. The beta-glucan presented high inhibition of virus replication by plaque assay (83.2%) and by immunofluorescence assay (63.8%). Although the mechanism has yet to be defined, we suggest that PLS and beta-glucan inhibited BoHV-1 replication by interfering with the early events of viral penetration. Additional studies are. required for a better understanding of the mechanism of action of PLS and beta-glucan.

• 出版日期2011