Limited availability of ZBP1 restricts axonal mRNA localization and nerve regeneration capacity

作者：Donnelly Christopher J; Willis Dianna E; Xu Mei; Tep Chhavy; Jiang Chunsu; Yoo Soonmoon; Schanen N Carolyn; Kirn Safran Catherine B; van Minnen Jan; English Arthur; Yoon Sung Ok; Bassell Gary J; Twiss Jeffery L^*

来源：The EMBO Journal, 2011, 30(22): 4665-4677.

DOI：10.1038/emboj.2011.347

• axon growth
• mRNA transport
• RNA binding protein
• nerve regeneration
• translation

Subcellular localization of mRNAs is regulated by RNA-protein interactions. Here, we show that introduction of a reporter mRNA with the 30UTR of beta-actin mRNA competes with endogenous mRNAs for binding to ZBP1 in adult sensory neurons. ZBP1 is needed for axonal localization of beta-actin mRNA, and introducing GFP with the 30UTR of beta-actin mRNA depletes axons of endogenous beta-actin and GAP-43 mRNAs and attenuates both in vitro and in vivo regrowth of severed axons. Consistent with limited levels of ZBP1 protein in adult neurons, mice heterozygous for the ZBP1 gene are haploinsufficient for axonal transport of beta-actin and GAP-43 mRNAs and for regeneration of peripheral nerve. Exogenous ZBP1 can rescue the RNA transport deficits, but the axonal growth deficit is only rescued if the transported mRNAs are locally translated. These data support a direct role for ZBP1 in transport and translation of mRNA cargos in axonal regeneration in vitro and in vivo. The EMBO Journal (2011) 30, 4665-4677. doi: 10.1038/emboj.2011.347; Published online 30 September 2011

• 出版日期2011-11-16