Molecular characterization, phylogeny analysis and pathogenicity of a Muscovy duck adenovirus strain isolated in China in 2014

作者：Zhang, Xinheng; Zhong, Yangjin; Zhou, Zhenhai; Liu, Yang; Zhang, Huanmin; Chen, Feng; Chen, Weiguo; Xie, Qingmei^*

来源：Virology, 2016, 493: 12-21.

DOI：10.1016/j.virol.2016.03.004

• Duck adenovirus 3
• Virus particles
• Physicochemical properties
• Sequence analysis
• Pathogenicity

This study aimed to characterize a novel adenovirus (AdV) isolated from diseased Muscovy ducks in China. After the AdV was successfully propagated in duck embryo fibroblasts, the morphological and physicochemical properties of the virions were studied by electron microscopy and different tests. The results of the analyses were in conformity with AdV properties. The full genome sequence was determined and analyzed. The new isolate (named CH-GD-12-2014) shared over 91% sequence identity with duck AdV-2 representing the species Duck aviadenovirus B. The most important distinguishing feature between the two DAdV strains was the presence of a second fiber gene in the Chinese isolate. Phylogeny reconstruction confirmed the affiliation of the virus with goose and duck AdVs in the genus Aviadenovirus. Experimental infection resulted in embryo death, and intramuscular inoculation provoked morbidity and mortality among ducks and chickens.

• 出版日期2016-6
• 单位华南热带农业大学; 华南农业大学