Dietary effect of selenium-fortified Chlorella vulgaris on reproduction of Brachionus plicatilis species complex (Rotifera: Monogononta)

作者：Kim Hee Jin^*; Nakamura Koh; Hagiwara Atsushi

来源：International Review of Hydrobiology, 2014, 99(1-2): 161-165.

DOI：10.1002/iroh.201301718

• Diet augmentation
• Population growth
• Sexual reproduction
• Brachionidae

We investigated the effects of fortifying a diet of Chlorella vulgaris with Selenium (Se) on sexual and asexual reproduction of rotifers in the Brachionus plicatilis species complex: i.e. two strains of B. plicatilis sensu stricto and one of Brachionus rotundiformis. These rotifers were cultured for 8-10 days on one of three different diets that were adjusted to provide the same dry weight of food: non-fortified Chlorella, Se-fortified Chlorella, and Nannochloropsis oculata. B. plicatilis (Makishima strain), which is obligatorily asexual, showed no difference in population growth rate among the three different diets (r = 0.55-0.61). On the other hand, B. plicatilis (NH17L strain), which reproduces by cyclical parthenogenesis, showed higher population growth (r = 0.25) and also higher rates of fertilization (35.9%) and absolute resting egg production (2803.9 eggs/g food) with the Se-fortified Chlorella diet than with other foods. Although B. rotundiformis (Kochi strain), which also exhibits cyclical parthenogenesis, showed no differences in population growth among the three different diets (r = 0.42-0.48), sexual reproduction parameters were different depending on the feeding regime. The highest mixis (26.2%), fertilization (72.6%), and resting egg production (3489.9 eggs/g food) were observed with the Se-fortified Chlorella diet. We posit that the effect of Se-fortified diet was greater on the resting egg production by enhancing male fertility than on population growth.

• 出版日期2014-3