A method of chemiluminescence coupled with ultrafiltration for investigating the interaction between ibuprofen and human serum albumin

作者：Xiong Xunyu^*; Zhang Qunzheng; Nan Yefei; Gu Xuefan

来源：Luminescence, 2013, 28(6): 954-960.

DOI：10.1002/bio.2465

• chemiluminescence
• ultrafiltration
• ibuprofen
• human serum albumin

In acidic media, ibuprofen substantially enhanced the weak chemiluminescence (CL) produced by sodium sulfite and potassium permanganate. The increased signals were linearly correlated with ibuprofen concentrations ranging from 1.2x10(-3) to 4.8M, with a detection limit of 4.8x10(-4)M. Two ultrafiltration (UF) membranes were used to construct a unit for trapping 0.15 and 0.75M human serum albumin (HSA) and coupled online with the CL system. At low HSA concentrations, the numbers of bound molecules per binding site were calculated to be 0.9 for Sudlow site I and 6.2 for Sudlow site II. The association constants on these binding sites were 5.9x10(5) and 3.4x10(4)M(-1), respectively. Our CL-UF protocol presents a rapid and sensitive method for studies on drug-protein interaction.

• 出版日期2013-11
• 单位西安石油大学