Elucidation of Antifungal Metabolites Produced by Pseudomonas aurantiaca IB5-10 with Broad-Spectrum Antifungal Activity

作者：Park Gwee Kyo; Lim Jong Hui; Kim Sang Dal; Shim Sang Hee^*

来源：Journal of Microbiology and Biotechnology, 2012, 22(3): 326-330.

DOI：10.4014/jmb.1106.06042

• Pseudomonas aurantiaca
• antifungal activities
• phenazine-1-carboxylic acid (PCA)
• 2-hydroxyphenazine (2-OH-PHZ)
• cyclo-(L-Pro-L-Val)

Antifungal metabolites were isolated from a culture of Pseudomonas aurantiaca IBS-10. Chemical structures of the metabolites were elucidated as phenazine-1-carboxylic acid (PCA; 1), 2-hydroxyphenazine (2-OH-PHZ; 2), and cyclo-(L-Pro-L-Val; 3), respectively, based on spectroscopic methods. Among them, 3 was isolated for the first time from this strain. The antifungal activities of 1-3 were evaluated against a variety of plant pathogens. To the best of our knowledge, the antifungal activities of 3 against plant fungal pathogens have been evaluated for the first time in this work. PCA (1) showed the most potent antifungal activities against Phytophthora capsici, Rhizoctonia solani AG-1(IA), and Pythium ultimum with MICs (mu g-ml) of less than 1.0, 1.3, and 2.0, respectively. On the other hand, 2-OH-PHZ (2) showed potent antifungal activity against R. solani AG-1(IA) with the MIC (mu g/ml) of 2.0, whereas it showed moderate antifungal activity against P. ultimum with the MIC (mu g/ml) of 50.0. In addition, 3 showed antifungal activity against only R. solani AG-1(IA).

• 出版日期2012-3