Directional tissue migration through a self-generated chemokine gradient

作者：Dona Erika; Barry Joseph D; Valentin Guillaume; Quirin Charlotte; Khmelinskii Anton; Kunze Andreas; Durdu Sevi; Newton Lionel R; Fernandez Minan Ana; Huber Wolfgang; Knop Michael; Gilmour Darren^*

来源：Nature, 2013, 503(7475): 285-+.

DOI：10.1038/nature12635

The directed migration of cell collectives is a driving force of embryogenesis(1-3). The predominant view in the field is that cells in embryos navigate along pre-patterned chemoattractant gradients(2). One hypothetical way to free migrating collectives from the requirement of long-range gradients would be through the self-generation of local gradients that travel with them(4,5), a strategy that potentially allows self-determined directionality. However, a lack of tools for the visualization of endogenous guidance cues has prevented the demonstration of such self-generated gradients in vivo. Here we define the in vivo dynamics of one key guidance molecule, the chemokine Cxcl12a, by applying a fluorescent timer approach to measure ligand-triggered receptor turnover in living animals. Using the zebrafish lateral line primordium as a model, we show that migrating cell collectives can self-generate gradients of chemokine activity across their length via polarized receptor-mediated internalization. Finally, by engineering an external source of the atypical receptor Cxcr7 that moves with the primordium, we show that a self-generated gradient mechanism is sufficient to direct robust collective migration. This study thus provides, to our knowledge, the first in vivo proof for self-directed tissue migration through local shaping of an extracellular cue and provides a framework for investigating self-directed migration in many other contexts including cancer invasion(6).

• 出版日期2013-11-14