Mild hypothermia effects on intercellular adhesion molecule-1 and serum interleukin-6 expression in brain tissues of a rat focal ischemia model

作者：Fu Shengqi^*; Yang Lei; Zhang Shuling; Sun Shilong; Mao Xingai

来源：Neural Regeneration Research, 2008, 3(10): 1124-1127.

• cerebral ischemia
• intercellular adhesion molecule-1
• interleukin-6
• mild hypothermia

BACKGROUND: Previous studies have confirmed the neuroprotective effect of mild hypothermia on ischemic brain injury.
OBJECTIVE: To investigate the effects of mild hypothermia on intercellular adhesion molecule-1 expression and serum interleukin-6 levels in ischemic brain tissues of focal brain ischemia rats. and to explore the neuroprotective effects of mild hypothermia on ischemic brain injury.
DESIGN, TIME AND SETTING: A randomized, controlled. neurobiological experiment was performed at the Central Laboratory, First Affiliated Hospital, Xinxiang Medical College, China from February to July 2006.
MATERIALS: Thirty healthy, adult. Sprague Dawley rats were used to establish middle cerebral artery occlusion models using the suture method. The immunohistochemistry (streptavidin-biotin-peroxidase complex method) kit was purchased from Boster, China. Interleukin-6 radioimmunoassay was supplied by Institute of Radioimmunity. Technology Development Center, General Hospital of Chinese PLA.
METHODS: The rats were equally and randomly assigned into mild hypothermia and control groups. and middle cerebral artery Occlusion models were established. The rectal temperature was maintained at (37 +/- 0.5) degrees C in the control group. In the mild hypothermia group, the rectal temperature was maintained at (33 +/- 1) degrees C.
MAIN OUTCOME MEASURES: At 12 hours after model establishment. the ischemic brain hemispheres were coronally sliced at the level of the optic chiasm. The number of intercellular adhesion molecule-1-positive vessels per high-power field was observed with an optical microscope. Serum interleukin-6 levels were measured by radioimmunoassay.
RESULTS: Compared with the control group, intercellular adhesion molecule-1 and serum interleukin-6 expressions were significantly decreased in ischemic brain tissues of the mild hypothermia group (P < 0.01).
CONCLUSION: Mild hypothermia exhibits a neuroprotective effect by reducing serum interleukin-6 and intercellular adhesion molecule-1 expression following cerebral ischemia.

• 出版日期2008-10
• 单位河南省人民医院; 新乡医学院