ASPicDB: a database of annotated transcript and protein variants generated by alternative splicing

作者：Martelli Pier L; D'Antonio Mattia; Bonizzoni Paola; Castrignano Tiziana; D'Erchia Anna M; De Meo Paolo D'Onorio; Fariselli Piero; Finelli Michele; Licciulli Flavio; Mangiulli Marina; Mignone Flavio; Pavesi Giulio; Picardi Ernesto; Rizzi Raffaella; Rossi Ivan; Valletti Alessio; Zauli Andrea; Zambelli Federico; Casadio Rita; Pesole Graziano^*

来源：Nucleic Acids Research, 2011, 39(Database): D80-D85.

DOI：10.1093/nar/gkq1073

Alternative splicing is emerging as a major mechanism for the expansion of the transcriptome and proteome diversity, particularly in human and other vertebrates. However, the proportion of alternative transcripts and proteins actually endowed with functional activity is currently highly debated. We present here a new release of ASPicDB which now provides a unique annotation resource of human protein variants generated by alternative splicing. A total of 256 939 protein variants from 17 191 multi-exon genes have been extensively annotated through state of the art machine learning tools providing information of the protein type (globular and transmembrane), localization, presence of PFAM domains, signal peptides, GPI-anchor propeptides, transmembrane and coiled-coil segments. Furthermore, full-length variants can be now specifically selected based on the annotation of CAGE-tags and polyA signal and/or polyA sites, marking transcription initiation and termination sites, respectively. The retrieval can be carried out at gene, transcript, exon, protein or splice site level allowing the selection of data sets fulfilling one or more features settled by the user. The retrieval interface also enables the selection of protein variants showing specific differences in the annotated features. ASPicDB is available at http://www.caspur.it/ASPicDB/.

• 出版日期2011-1