An improved cell isolation method for flow cytometric and functional analyses of cutaneous wound leukocytes

作者:Brubaker Aleah L; Schneider David F; Palmer Jessica L; Faunce Douglas E; Kovacs Elizabeth J*
来源:Journal of Immunological Methods, 2011, 373(1-2): 161-166.
DOI:10.1016/j.jim.2011.08.013

摘要

Isolation of leukocytes from full-thickness excisional wounds has proven to be a difficult process that results in poor cell yield and holds significant limitations for functional assays. Given the increased interest in the isolation, characterization and functional measurements of wound-derived cell populations, herein we describe a method for preparing wound cell suspensions with an improved yield that enables both phenotypic and functional assessments.

  • 出版日期2011-10-28