A pilot pharmacokinetic study of miroestrol and deoxymiroestrol on rabbit sera using polyclonal antibody-based icELISA analysis

作者：Kitisripanya Tharita; Udomsin Orapin; Komaikul Jukrapun; Inyai Chadathorn; Limsuwanchote Supattra; Yusakul Gorawit; Putalun Wara****^*

来源：Phytotherapy Research, 2018, 32(2): 365-369.

DOI：10.1002/ptr.5991

• deoxymiroestrol
• immunoassay
• miroestrol
• pharmacokinetic
• Pueraria candollei var. mirifica Airy Shaw & Suvat

Miroestrol (ME) and deoxymiroestrol (DME) are the most potent phytoestrogens and bioactive markers in Pueraria candollei var. mirifica tuberous roots. To understand their pharmacokinetic profiles, a pharmacokinetic study of ME and DME, at 0.43 and 0.21 mg per kg body weight, respectively, in three rabbits was performed after orally administering a single dose of P. candollei var. mirifica enriched fraction extract. Two established polyclonal antibody-based indirect competitive enzyme-linked immunosorbent assays were validated to determine ME and DME in rabbit sera. In rabbits, the area under the 0- to 48-hr concentration-time curve of ME and DME were 854.92 and 1,692.84 ng.h/ml, respectively. The maximum concentration of ME was measured 1 hr after administration as 69.62 +/- 8.28 ng/ml, and the maximum concentration of DME was measured at 3 hr as 81.8 +/- 5.43 ng/ml. These results provide an initial approach for designing and studying the relationship between the ME and DME levels and their therapeutic effects based on their pharmacokinetic profiles.

• 出版日期2018-2