A Versatile Scaffold Contributes to Damage Survival via Sumoylation and Nuclease Interactions

作者：Sarangi Prabha; Altmannova Veronika; Holland Cory; Bartosova Zdenka; Hao Fanfan; Anrather Dorothea; Ammerer Gustav; Lee Sang Eun; Krejci Lumir^*; Zhao Xiaolan

来源：Cell Reports, 2014, 9(1): 143-152.

DOI：10.1016/j.celrep.2014.08.054

DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes in recognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing a particular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects of Saw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.

• 出版日期2014-10-9