Low level laser therapy reduces acute lung inflammation in a model of pulmonary and extrapulmonary LPS-induced ARDS

作者：Oliveira Manoel Carneiro Jr; Greiffo Flavia Regina; Rigonato Oliveira Nicole Cristine; Alberca Custodio Ricardo Wesley; Silva Vanessa Roza; Damaceno Rodrigues Nilsa Regina; Almeida Francine Maria; Albertini Regiane; Lopes Martins Rodrigo Alvaro B; Franco de Oliveira Luis Vicente; Camillo de Carvalho Paulo de Tarso; Ligeiro de Oliveira Ana Paula; Leal Ernesto Cesar P Jr; Vieira Rodolfo P^*

来源：Journal of Photochemistry and Photobiology B: Biology , 2014, 134: 57-63.

DOI：10.1016/j.jphotobiol.2014.03.021

• ARDS
• LPS
• LLLT
• Lung inflammation
• Cytokines
• Bronchoalveolar lavage

The present study aimed to investigate the effects low level laser therapy (LLLT) in a LPS-induced pulmonary and extrapulmonary acute respiratory distress syndrome (ARDS) in BALB/c mice. Laser (830 nm laser, 9 J/cm(2), 35 mW, 80 s per point, 3 points per application) was applied in direct contact with skin, 1 h after LPS administration. Mice were distributed in control (n = 6; PBS), ARDS IT (n = 7; LPS orotracheally 10 mu g/mouse), ARDS IP (n = 7; LPS intra-peritoneally 100 mu g/mouse), ARDS IT + Laser (n = 9; LPS intra-tracheally 10 mu g/mouse), ARDS IP + Laser (n = 9; LPS intra-peritoneally 100 mu g/mouse). Twenty-four hours after last LPS administration, mice were studied for pulmonary inflammation by total and differential cell count in bronchoalveolar lavage (BAL), cytokines (IL-1 beta, IL-6, KC and TNF-alpha) levels in BAL fluid and also by quantitative analysis of neutrophils number in the lung parenchyma. LLLT significantly reduced pulmonary and extrapulmonary inflammation in LPS-induced ARDS, as demonstrated by reduced number of total cells (p < 0.001) and neutrophils (p < 0.001) in BAL, reduced levels of IL-1 beta, IL-6, KC and TNF-alpha in BAL fluid and in serum (p < 0.001), as well as the number of neutrophils in lung parenchyma (p < 0.001). LLLT is effective to reduce pulmonary inflammation in both pulmonary and extrapulmonary model of LPS-induced ARDS.

• 出版日期2014-5-5