Proteomic analysis of the rat cerebellar flocculus during vestibular compensation

作者：Fukasawa Masahiko; Okamoto Kazuki^*; Nakamura Manabu; Mikami Koshi; Shimada Sonoko; Tanaka Yasuhiko; Nagai Kouhei; Arito Mitsumi; Kurokawa Manae S; Masuko Kayo; Suematsu Naoya; Koizuka Izumi; Kato Tomohiro

来源：Journal of Vestibular Research-Equilibrium & Orientation, 2009, 19(3-4): 83-94.

DOI：10.3233/VES-2009-0356

• Unilateral labyrinthectomy
• vestibular compensation
• cerebellar flocculus
• proteomics

Unilateral labyrinthectomy (UL) in rats is used as a human vertigo model. In this model, spontaneous nystagmus and dysequilibrium caused by UL are ameliorated within 48-72 hours. The amelioration, termed vestibular compensation (VC), is long lasting. Although cerebellar flocculi have been reported to be involved in VC, the molecular mechanisms behind VC are unknown. In this study, we used 2D-DIGE to detect protein changes in flocculi during acute (48 hours) and chronic (1 week) stages of VC. We found 99 out of 967 protein spots that showed significant changes in their intensities. Of the 99 spots, 45 spots (ipsilateral side, 15; contralateral side, 30) changed unilaterally during the acute stage, whereas 46 spots (ipsilateral side, 21; contralateral side, 25) changed unilaterally during the chronic stage. Thus, the acute compensation mechanism is more complicated in the contralateral flocculus than in the ipsilateral flocculus. Using MALDI-TOF MS, we identified 10 proteins out of the 12 protein spots. Of these, 3 proteins involved in synaptic transmission, neuronal filament formation and vesicular transport, respectively, demonstrated VC generation.

• 出版日期2009