Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions

作者：Ito Kenichiro; Liu Hongshan; Komiyama Makoto; Hayashi Tetsuya; Xu Yan^*

来源：Molecules, 2013, 18(10): 12909-12915.

DOI：10.3390/molecules181012909

• click chemistry
• cAMP
• fluorescence
• cAMP localization in living cells

8-Azidoadenosine 3%26apos;,5%26apos;-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.

• 出版日期2013-10