Level and value of circulating endothelial progenitor cells in patients with acute myocardial infarction undergoing primary coronary angioplasty: in vivo and in vitro studies

作者：Chang, Hsueh-Wen; Leu, Steve; Sun, Cheuk-Kwan; Hang, Chi-Ling; Youssef, Ali A.; Hsieh, Yuan-Kai; Yang, Cheng-Hsu; Cheng, Cheng-I; Chen, Shyh-Ming; Chen, Chien-Jen; Chua, Sarah; Chang, Li-Teh; Wu, Chiung-Jen; Yip, Hon-Kan^*

来源：Translational Research, 2010, 156(4): 251-263.

DOI：10.1016/j.trsl.2010.07.010

• BONE-MARROW
• ARTERY-DISEASE
• CLINICAL-OUTCOMES
• PERIPHERAL-BLOOD
• ISCHEMIC-STROKE
• HEART-FAILURE
• STEM-CELLS
• MOBILIZATION
• NUMBER
• NEOVASCULARIZATION

Levels of circulating endothelial progenitor cells (EPCs) in acute ST-elevation myocardial infarction (STEMI) patients undergoing primary coronary intervention (PCI) were investigated in this study. Flow cytometric analysis of the circulating EPC level (CD31/CD34 (E-1), CD62E/CD34 (E-2), and KDR/CD34 (E-3)) was determined from blood samples of 161 consecutive patients with STEMI undergoing primary PCI. Angiogenesis was evaluated using mononuclear cell-derived EPCs on Matrigel. The EPC number (E1-3) was lower in STEMI patients than in normal subjects (n = 25) (P < 0.005). Patients with high EPCs (E1-3) (>= 1.2%) had a lower left ventricular ejection fraction, elevated white blood cell count and creatinine level, advanced Killip score (>= class 3), more advanced congestive heart failure (CHF) (>= class 3), and increased 30-day mortality than those with a low EPC (E1-3) level (<1.2%) (P < 0.0001). Angiogenesis was lower in patients with a high EPC level than those with a low EPC level and normal controls (P < 0.001). Both the advanced Killip score and the CHF were independent predictors of increased EPC levels (P < 0.05). Multivariate analysis identified a high EPC (E-3) level to be the most important predictor of increased 30-day major adverse clinical outcome (MACO) (P < 0.0001). In conclusion, the circulating EPC level is a major independent predictor of 30-day MACO in patients with STEMI undergoing primary PCI. (Translational Research 2010;156:251-263)

• 出版日期2010-10
• 单位中山大学; 长春大学