Distinct mechanisms for DNA cleavage by myoglobin with a designed heme active center

作者：Zhao, Yuan; Du, Ke-Jie^*; Gao, Shu-Qin; He, Bo; Wen, Ge-Bo; Tan, Xiangshi; Lin, Ying-Wu^*

来源：Journal of Inorganic Biochemistry, 2016, 156: 113-121.

DOI：10.1016/j.jinorgbio.2016.01.004

• Heme proteins
• Protein design
• DNA cleavage
• Mechanism
• Hydrogen-bonding network

Heme proteins perform diverse biological functions, of which myoglobin (Mb) is a representative protein. In this study, the O-2 carrier Mb was shown to cleave double stranded DNA upon aerobic dithiothreitol-induced reduction, which is fine-tuned by an additional distal histidine, His29 or His43, engineered in the heme active center. Spectroscopic (UV-vis and EPR) and inhibition studies suggested that free radicals including singlet oxygen and hydroxyl radical are responsible for efficient DNA cleavage via an oxidative cleavage mechanism. On the other hand, L29E Mb, with a distinct heme active center involving three water molecules in the met form, was found to exhibit an excellent DNA cleavage activity that was not depending on O-2. Inhibition and ligation studies demonstrated for the first time that L29E Mb cleaves double stranded DNA into both the nicked circular and linear forms via a hydrolytic cleavage mechanism, which resembles native endonucleases. This study provides valuable insights into the distinct mechanisms for DNA cleavage by heme proteins, and lays down a base for creating artificial DNA endonucleases by rational design of heme proteins. Moreover, this study suggests that the diverse functions of heme proteins can be fine-tuned by rational design of the heme active center with a hydrogen bonding network.

• 出版日期2016-3
• 单位复旦大学; 南华大学