A noncoding RNA produced by arthropod-borne flaviviruses inhibits the cellular exoribonuclease XRN1 and alters host mRNA stability

作者：Moon Stephanie L; Anderson John R; Kumagai Yutaro; Wilusz Carol J; Akira Shizuo; Khromykh Alexander A; Wilusz Jeffrey

来源：RNA-A Publication of the RNA Society, 2012, 18(11): 2029-2040.

DOI：10.1261/rna.034330.112

• flavivirus
• mRNA decay
• mRNA stability
• noncoding RNA
• virus-host interaction

All arthropod-borne flaviviruses generate a short noncoding RNA (sfRNA) from the viral 3%26apos; untranslated region during infection due to stalling of the cellular 5%26apos;-to-3%26apos; exonuclease XRN1. We show here that formation of sfRNA also inhibits XRN1 activity. Cells infected with Dengue or Kunjin viruses accumulate uncapped mRNAs, decay intermediates normally targeted by XRN1. XRN1 repression also resulted in the increased overall stability of cellular mRNAs in flavivirus-infected cells. Importantly, a mutant Kunjin virus that cannot form sfRNA but replicates to normal levels failed to affect host mRNA stability or XRN1 activity. Expression of sfRNA in the absence of viral infection demonstrated that sfRNA formation was directly responsible for the stabilization of cellular mRNAs. Finally, numerous cellular mRNAs were differentially expressed in an sfRNA-dependent fashion in a Kunjin virus infection. We conclude that flaviviruses incapacitate XRN1 during infection and dysregulate host mRNA stability as a result of sfRNA formation.

• 出版日期2012-11