A novel method of silkworm embryo preparation for immunohistochemistry

作者：You Zaizhi; Sun Chunfeng; Chen Liang; Yao Qin; Chen Keping^*

来源：Biotechnology Letters, 2013, 35(8): 1209-1214.

DOI：10.1007/s10529-013-1202-x

• Embryo
• Immunohistochemistry
• Silkworm

It is difficult to obtain intact embryos, especially intact early embryos, from insect eggs because of their small sizes. Based on the means traditionally used to get silkworm embryos and the previous approaches used for getting Drosophila embryos, we established a novel method of silkworm embryo preparation. The new method is straightforward and easy to operate. Silkworm embryos could be prepared without severe damage in large quantities by this new protocol. In addition, the novel method of silkworm embryo preparation is quite suitable for immunohistochemistry.

• 出版日期2013-8
• 单位江苏大学