Automated system for on-line desorption of dried blood spots applied to LC/MS/MS pharmacokinetic study of flurbiprofen and its metabolite

作者：Deglon Julien; Thomas Aurelien; Daali Youssef; Lauer Estelle; Samer Caroline; Desmeules Jules; Dayer Pierre; Mangin Patrice; Staub Christian^*

来源：Journal of Pharmaceutical and Biomedical Analysis, 2011, 54(2): 359-367.

DOI：10.1016/j.jpba.2010.08.032

• On-line DBS
• Automated system
• LC/MS/MS
• Flurbiprofen
• Pharmacokinetic

This paper illustrates the development of an automated system for the on-line bioanalysis of dried blood spots (on-line DBS). In this way, a prototype was designed for integration into a conventional LC/MS/MS, allowing the successive extraction of 30 DBS toward the analytical system without any sample pretreatment.
The developed method was assessed for the DBS analysis of flurbiprofen (FLB) and its metabolite 4-hydroxyflurbiprofen (OH-FLB) in human whole blood (i.e. 5 mu L.). The automated procedure was fully validated based on international criteria and showed good precision, trueness, and linearity over the expected concentration range (from 10 to 1000 ng/mL and 100 to 10,000 ng/mL for OH-FLB and FLB respectively). Furthermore, the prototype showed good results in terms of recovery and carry-over. Stability of both analytes on filter paper was also investigated and the results suggested that DBS could be stored at ambient temperature for over 1 month.
The on-line DBS automated system was then successfully applied to a pharmacokinetic study performed on healthy male volunteers after oral administration of a single 50-mg dose of FLB. Additionally, a comparison between finger capillary DES and classic venous plasma concentrations was investigated. A good correlation was observed, demonstrating the complementarity of both sampling forms.
The automated system described in this article represents an efficient tool for the LC/MS/MS analysis of DBS samples in many bioanalytical applications.

• 出版日期2011-1-25