Angiogenin-induced tRNA-derived Stress-induced RNAs Promote Stress-induced Stress Granule Assembly

作者：Emara Mohamed M; Ivanov Pavel; Hickman Tyler; Dawra Nemisha; Tisdale Sarah; Kedersha Nancy; Hu Guo Fu; Anderson Paul^*

来源：JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285(14): 10959-10968.

DOI：10.1074/jbc.M109.077560

Angiogenin (ANG) is a secreted ribonuclease that cleaves tRNA to initiate a stress-response program in mammalian cells. Here we show that ANG inhibits protein synthesis and promotes arsenite-and pateamine A-induced assembly of stress granules (SGs). These effects are abrogated in cells transfected with the ANG inhibitor RNH1. Transfection of natural or synthetic 5'-but not 3'-tRNA fragments (tRNA-derived stress-induced RNAs; tiRNAs) induces the phospho-eukaryotic translation initiation factor 2 alpha-independent assembly of SGs. Natural 5'-tiRNAs but not 3'-tiRNAs are capped with a 5'-monophosphate that is required for optimal SG assembly. These findings reveal that SG assembly is a component of the ANG- and tiRNA-induced stress response program.

• 出版日期2010-4-2