A novel NBD-based fluorescent turn-on probe for the detection of cysteine and homocysteine in living cells

作者：Wang, Jiamin^*; Niu, Linqiang; Huang, Jing; Yan, Zhijie; Wang, Jianhong^*

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2018, 192: 52-58.

DOI：10.1016/j.saa.2017.10.064

• 7-nitro-2,1,3-benzoxadiazole (NBD)
• Fluorescent probe
• Cys/Hcy
• Normal cell
• Cancer cells

Biothiols, such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), are involved in a number of biological processes and play crucial roles in biological systems. Thus, the detection of biothiols is highly important for early diagnosis of diseases and evaluation of disease progression. Herein, we developed a new turn-on fluorescent probe 1 based on 7-nitro-2,1,3-benzoxadiazole (NBD) with high selectivity and sensitivity for Cys/Hcy on account of nucleophilic substitution and Smiles rearrangement reaction. The probe could sense Cys/Hcy rapidly, the intensity of fluorescence increased immediately within 1 min. Furthermore, the probe is low toxic and has been successfully applied to detect intracellular Cys/Hcy by cell fluorescence imaging in living normal and cancer cells.

• 出版日期2018-3-5
• 单位河南大学