Anti-tumor effects of Gecko ethanol extract on human cervical cancer SiHa cells

作者：Ge, Wen-Jing; Liu, Ling; Li, Rui-Fang; Duan, Leng-Xin; Liu, Hong-Chao; Wang, Jian-Gang^*

来源：International Journal of Clinical and Experimental Medicine, 2016, 9(10): 18962-18971.

• Gecko ethanol extract
• human cervical cancer
• anti-tumor
• apoptosis

Gecko has been applied as an anti-tumor agent in traditional Chinese medicine for hundreds of years. Gecko ethanol extract (GEE) which was extracted from the powder of whole Gecko, has shown the obvious antitumor activity. It is still unclear the effect of GEE on human cervical cancer cells. In this study we investigated the antitumor effect of GEE on human cervical cancer SiHa cells and its potential molecular mechanism. The growth inhibitory effect of GEE on SiHa cells were assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay and colony forming assay in vitro. The results showed that GEE significantly inhibited proliferation of SiHa cells in a dose-and time-dependent manners. The typical morphological changes of apoptosis were observed in SiHa cells, such as chromatin condensation, nuclear fragmentation and reduction of cell volume. The effects of GEE on proteins expression of SiHa cells were detected by Western blot assay. The protein expression levels of Bcl-2 and VEGF were down-regulated, whereas Bax, cytochrome c (Cyt c), caspase-9 and caspase-3 protein were up-regulated after treatment of GEE in vitro. In a word, these results suggested that GEE play an antineoplastic role on SiHa cells, which may be concerned with the effect of anti-proliferation, anti-angiogenesis and apoptosis-induction.

• 出版日期2016
• 单位河南大学