Alpinia nigra seeds: A potential source of free radical scavenger and antibacterial agent

作者：Ghosh Sudipta; Padilla Gonzalez Guillermo F; Rangan Latha^*

来源：Industrial Crops and Products, 2013, 49: 348-356.

DOI：10.1016/j.indcrop.2013.05.002

• Alpinia nigra
• Antibacterial activity
• DPPH assay
• Flow cytometry
• Minimum inhibitory concentration

The radical scavenging activity and the antibacterial properties of different solvent (hexane, ethyl acetate and methanol) extracts of Alpinia nigra seeds were investigated in the present study. All the extracts were used to assess their potential antioxidant activities using methods for scavenging of 2,2-diphenyl-1-picrylhydrazyl radical. Ethyl acetate and methanol extracts exhibited effective free radical scavenging activities compared to the standard antioxidant butylated hydroxyl toluene. The efficacy of A. nigra seed extracts was tested against three gram positive and four gram negative bacteria. Flow cytometry and field emission scanning electron microscopy study reveals and confirms the bacterial cell membrane damage, pore formation and membrane depolarization when treated with different solvent extracts. Bacterial cell membrane damage and releasing of cytoplasmic content (nucleic acids) was monitored using UV/vis spectrophotometer at 260 nm. Current investigation highlights the antimicrobial potential of A. nigra seed extracts and its total antioxidant efficacy for the first time.

• 出版日期2013-8