Detection of NAD(P)H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes

作者：Ito Hiroki; Terai Takuya^*; Hanaoka Kenjiro; Ueno Tasuku; Komatsu Toru; Nagano Tetsuo; Urano Yasuteru

来源：Chemical Communications, 2015, 51(39): 8319-8322.

DOI：10.1039/c5cc01613d

We discovered that positively charged terbium complexes bearing 1,4,7,10-tetraazacyclododecane functionalized with amide ligands are highly sensitive to dynamic luminescence quenching by NAD(P)H. We exploited this phenomenon to establish a general time-resolved luminescence-based assay platform for sensitive detection of NAD(P)H-dependent enzyme activities.

• 出版日期2015