An evaluation of the applicability of microarrays for monitoring toxic algae in Irish coastal waters

作者：McCoy Gary R^*; Touzet Nicolas; Fleming Gerard T A; Raine Robin

来源：Environmental Science and Pollution Research, 2013, 20(10): 6751-6764.

DOI：10.1007/s11356-012-1294-1

• Microarrays
• Hierarchical probes
• Ribosomal RNA
• HABs
• Environmental monitoring
• Cell concentrations

The applicability of microarrays to monitor harmful algae across a broad range of ecological niches and toxic species responsible for harmful algal events has been one of the key tasks in the EU Seventh Framework Programme (FP7)-funded Microarrays for the Detection of Toxic Algae project. The technique has a strong potential for improving speed and accuracy of the identification of harmful algae and their toxins to assist monitoring programmes. Water samples were collected from a number of coastal sites around Ireland, including several that are used in the Irish National Phytoplankton and Biotoxin Monitoring Programme. Ribosomal RNA was extracted from filtered field samples, labelled with a fluorescent dye, and hybridised to probes spotted in a microarray format on a glass slide. The fluorescent signal intensity of the hybridisation to > 120 probes on the chip was analysed and compared with actual field counts. There was a general agreement between cell counts and microarray signal. Results are presented for field samples taken from a range of stations along the Irish coastline known for harmful algal events during the first field trial (July 2009-April 2010).

• 出版日期2013-10