Genetic diversity of the endangered Faucaria tigrina (Aizoaceae) through ISSR "fingerprinting" using automated fragment detection

作者：Bentley Luke; Barker Nigel P^*; Dold Anthony P

来源：Biochemical Systematics and Ecology, 2015, 58: 156-161.

DOI：10.1016/j.bse.2014.11.012

• Aizoaceae
• Automated detection
• Faucaria tigrina
• Genetic diversity
• ISSR fingerprinting
• Conservation genetics

Faucaria tigrina (Haw.) Schwantes is a rare, threatened succulent found exclusively on the outskirts of Grahamstown, Eastern Cape, South Africa. There are three extant populations of the species; two large ones (approximately 300 and 620 adults) separated by under two kilometres, and a third very much smaller one slightly further away. Genetic theory expects that smaller, isolated populations face a loss in genetic variation through inbreeding and genetic drift and that as declining genetic variation is linked to a loss in fitness, this species may face extinction in the long term. This study used the ISSR-PCR genetic methods linked to an automated detection platform to determine if these populations are genetically distinct, and whether they are genetically depauperate. Two ISSR primers were used, and the automated detection system identified a total of 572 ISSR loci. An UPGMA clustering analysis showed each population to be genetically distinct, and that the genetic diversity of each population does not appear to be particularly low. E tigrina is separable from the related Faucaria britteniae L. Bolus using the ISSR method, suggesting that this method may be appropriate for systematic studies in the Aizoaceae, which comprises many genera with closely related species.

• 出版日期2015-2