A trifunctional cyclooctyne for modifying azide-labeled biomolecules with photocrosslinking and affinity tags

作者：Stewart Jessica A; Piligian Brent F; Rundell Sarah R; Swarts Benjamin M^*

来源：Chemical Communications, 2015, 51(99): 17600-17603.

DOI：10.1039/c5cc07536j

A bicyclo[6.1.0]nonyne (BCN)-based cyclooctyne reagent bearing a photocrosslinking diazirine (DAz) group and a biotin affinity handle, BCN-DAz-Biotin, is reported. BCN-DAz-Biotin is capable of simultaneously delivering photocrosslinking and affinity tags to azide-labeled biomolecules, enabling photoactivated capture and enrichment/detection of interacting species in native contexts.

• 出版日期2015