Toll-like receptor 4 is involved in myocardial damage following paraquat poisoning in mice

作者：Dong Xue Song; Xu Xiao Yang; Sun Yu Qiang; Wei Liu; Jiang Zhe Hui; Liu Zhi^*

来源：Toxicology, 2013, 312: 115-122.

DOI：10.1016/j.tox.2013.08.009

• Paraquat
• Toxicity
• Cardiomyocytes
• Pathology
• Toll-like receptor 4
• Tumor necrosis factor-alpha
• Interleukin-1 beta

The ingestion of the herbicide paraquat (PQ) can cause multiple organ injury including cardiac lesions. However, the underlying mechanism of myocardial damage is not known. Toll-like receptor 4 (TRL4) is a pattern-recognition receptor in the innate immune response to microbial pathogens. TLR4 is involved in heart dysfunction such as septic shock or myocardial ischemia. We investigated whether TLR4 would be linked to the pathogenesis of heart disease due to PQ exposure. Wild type mice (WT) and TLR4-deficient mice were injected intraperitoneally with 75 mg/kg of PQ to induce myocardial damage and tested for echocardiographic assessment, histopathology, pro-inflammatory cytokine and TLR4 expression. WT mice after PQ exposure displayed deteriorate cardiac function, pathological damages, increased TLR4 mRNA and protein levels as well as myocardial TNF-alpha and IL-beta levels. Compared with WT mice, TLR4-deficient mice were significantly resistant to the PQ-induced injury. We concluded that the TLR4 was required as a mediator and played an important role in myocardial damage due to PQ.

• 出版日期2013-10-4
• 单位中国医科大学