科研之友
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摘要
It is an important means to record rat hippocampal field potential, especially in vivo recording, in the study of learning and memory. To overcome the disadvantages and inconvenience in current hippocampal field potential recording and raise in vivo experimental efficiency, stimulation/recording/drug delivery system for in vivo hippocampal field potential recording was developed and applied. Anesthetized SD rats were fixed in a stereotaxic device and a homemade stimulation/recording/drug delivery system was used to record field excitatory postsynaptic potential (fEPSP) in hippocampal CA1 area. The results showed that fEPSPs were easily evoked by nearly every test stimulus and maintained steadily for a long time by using the stimulation/recording/drug delivery system; high frequency stimulation (HFS) successfully induced early-phase LTP (E-LTP) and late-phase LTP (L-LTP); the baseline fEPSPs and LTP were promptly and effectively suppressed by intrahippocampal injection (IH) of AMPA receptor antagonist CNQX (100 mu mol/L, 1 mu 1) and NMDA receptor antagonist AP-5 (100 mu mol/L, 1 mu 1), respectively, with a shorter latency and a reduced dose compared with intracerebraventricular injection (ICV); the induction and recording of paired pulse facilitation (PPF) were also considerably easy and stable using the combination device. In short, the simplicity, reliability and high efficiency of stimulation/recording/drug delivery combination system in recording in vivo rat hippocampal CA1 area field potential make it more practical in the research of synaptic plasticity, and this technique established an important foundation for the study of higher cognitive brain function.
- 出版日期2011-4
- 单位山西医科大学
