A chimeric multi-epitope DNA vaccine elicited specific antibody response against severe acute respiratory syndrome-associated coronavirus which attenuated the virulence of SARS-CoV in vitro

作者：Wang Xiaohua; Xu Wei; Tong Deyan; Ni Jing; Gao Haifeng; Wang Ying; Chu Yiwei; Li Pingping; Yang Xiaoming; Xiong Sidong^*

来源：Immunology Letters, 2008, 119(1-2): 71-77.

DOI：10.1016/j.imlet.2008.04.005

• SARS-CoV
• epitope
• DNA vaccine
• antibody
• prime-boost

Epitope-based vaccines designed to induce antibody responses specific for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) are being developed as a means for increasing vaccine potency. In this study, we identified four B cell epitopes from the spike (S) and membrane (M) protein through bioinformatics analysis and constructed a multi-epitope DNA vaccine. Intramuscular immunization of mice with this vaccine was sufficient to induce specific prime as well as a long-term memory humoral immune response to at least two candidate epitopes, S(437-459) and M(1-20). A DNA prime-protein boost strategy greatly enhanced the antibody generation and the immune sera not only reacted with the lysates of SARS-CoV-infected Vero cells but also neutralized the cytopathic effect of SARS by 75% at 1: 160 dilution. The novel immunogenic S protein peptide revealed in this study provides new target for SARS vaccine design; and our work indicated multi-epitope DNA vaccine as an effective means for eliciting polyvalent humoral immune response against SARS-CoV.

• 出版日期2008-8-15
• 单位复旦大学