Optimization of the refolding of recombinant human granulocyte-macrophage colony-stimulating factor immobilized on affinity sorbent

作者：Kosobokova E N^*; Skrypnik K A; Pinyugina M V; Shcherbakov A I; Kosorukov V S

来源：APPLIED BIOCHEMISTRY AND MICROBIOLOGY, 2014, 50(8): 773-779.

DOI：10.1134/S0003683814080031

• GM-CSF
• on-column refolding
• His-domain

A method for the production and purification of biologically active recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF), expressed in Escherichia coli, has been developed. Washing of an inclusion body fraction which allows the removal of numerous ballast proteins and on-column refolding of rhGM-CSF are specific characteristics of the method. The refolding efficiency reached 70%; the purity of the preparation constituted 95%. The biological activity was similar to those of other recombinant hGM-CSF analogs.

• 出版日期2014-12