Goat red blood cells as precursor for iron oxide nanocrystal synthesis to develop nuclear targeted lung cancer therapy

作者：Sreevani Vellingiri; Shanthi Krishnamurthy; Kannan Soundarapandian^*

来源：Materials Research Bulletin, 2013, 48(9): 3164-3171.

DOI：10.1016/j.materresbull.2013.04.079

• Metals
• Oxides
• Chemical synthesis
• X-ray diffraction
• Crystal structure

In this study, we synthesised iron oxide nanocrystals (Fe2O3-NC) from goat blood (bio-precursor) using red blood cells (RBC) lysis method (a molecular level approach) for the first time. The formation of Fe2O3-NC was achieved through a single-phase chemical reduction method. The size distribution of Fe2O3-NC falls between 20-30 nm for pellet and 100-200 nm for lysate and were found to be crystalline. Fe2O3-NC demonstrated significant cytotoxicity on A549. We report the direct visualization of interactions between Fe2O3-NC and the cancer cell nucleus. The active transport of Fe2O3-NC to the nucleus induces major changes to nuclear phenotype via nuclear envelope invaginations. We further examined the root cause for the involvement of Fe2O3-NC on the expression of caspase-3, caspase-7 and Bcl-2 in A549 cancer cells. This functional proteomic analysis clearly implies that the lung cancer cell proliferation is perfectly targeted by the biosynthesised Fe2O3-NC which could provide new insight for nuclear-targeted cancer therapy.

• 出版日期2013-9