Systems biology approach predicts immunogenicity of the yellow fever vaccine in humans

作者：Querec Troy D; Akondy Rama S; Lee Eva K; Cao Weiping; Nakaya Helder I; Teuwen Dirk; Pirani Ali; Gernert Kim; Deng Jiusheng; Marzolf Bruz; Kennedy Kathleen; Wu Haiyan; Bennouna Soumaya; Oluoch Herold; Miller Joseph; Vencio Ricardo Z; Mulligan Mark; Aderem Alan; Ahmed Rafi; Pulendran Bali^*

来源：Nature Immunology, 2009, 10(1): 116-125.

DOI：10.1038/ni.1688

A major challenge in vaccinology is to prospectively determine vaccine efficacy. Here we have used a systems biology approach to identify early gene 'signatures' that predicted immune responses in humans vaccinated with yellow fever vaccine YF-17D. Vaccination induced genes that regulate virus innate sensing and type I interferon production. Computational analyses identified a gene signature, including complement protein C1qB and eukaryotic translation initiation factor 2 alpha kinase 4-an orchestrator of the integrated stress response-that correlated with and predicted YF-17D CD8(+) T cell responses with up to 90% accuracy in an independent, blinded trial. A distinct signature, including B cell growth factor TNFRS17, predicted the neutralizing antibody response with up to 100% accuracy. These data highlight the utility of systems biology approaches in predicting vaccine efficacy.

• 出版日期2009-1